Free Vaccine Design Methods and Protocols, Volume 2 PDF Download

Free Vaccine Design Methods and Protocols, Volume 2 PDF Download

This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 2:Vaccines for Veterinary Diseases includes vaccines for farm animals and fishes, vaccine vectors and production, vaccine delivery systems, vaccine bioinformatics, vaccine regulation and intellectual property. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases aims to ensure successful results in the further study of this vital field.

Vaccinations have helped in preventing several diseases; however, as yet, there are only two
diseases that have been eradicated globally. Mass awareness programs and aggressive vaccination strategies in the twentieth century were able to control smallpox, and the disease
was officially declared eradicated in 1980. Rinderpest, a serious disease of cattle, was officially eradicated in 2011, thereby becoming only the second disease to be completely eradicated. Recently, the Americas (North and South America) were declared free of endemic
transmission of rubella, a contagious viral disease that can cause multiple birth defects as
well as fetal death when contracted by women during pregnancy. The achievement was due
to a 15-year effort that involved widespread administration of the vaccine against measles,
mumps, and rubella (MMR) throughout the Western Hemisphere.
One of the dreaded diseases—poliomyelitis—is in the last phases of eradication, thanks
to the effective vaccines against the disease. The public health effort to eliminate poliomyelitis infection around the world began in 1988, and vaccination strategies have reduced the
number of annual diagnosed cases of polio from the hundreds of thousands to couple of
hundreds. Nigeria was the last country in Africa to eradicate polio; as of writing this book,
no polio is reported in Nigeria since last year. Currently, polio remains endemic in two
countries—Afghanistan and Pakistan. Until poliovirus transmission is interrupted in these
countries, all other countries remain at risk of importation of polio. Illiteracy, ignorance to
vaccines, death threats, as well as killing of healthcare workers providing polio vaccines have
slowed immunization programs in Pakistan. This toxic scenario coupled with the migration
of people has led to the persistence of polio in Pakistan and neighboring Afghanistan. With
awareness for the need of vaccination, knowledge on the importance of vaccination, and
new rules that may penalize resistance to vaccination, it may be possible to eliminate polio
by the end of the decade.
When I was given the opportunity to author this book (Vaccine Design: Methods and
Protocols), I wished to have at least one chapter on vaccine design or vaccine development
from every country. Unfortunately, it dawned on me later that not every country invests in
science! It was also unfortunate to realize that research and development on vaccines is not
a priority even in some developed countries with resources or influence. New sustainable
technologies are to be developed to create more jobs and improve the well-being of humans
as well as conservation of nature; hence it is high time countries invest at least 5 % of their
GDP for science including vaccine development.

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